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代謝組學和轉錄組學關聯解析德欽紫花苜蓿根部應答高溫脅迫的調控機制

批準號31660683 學科分類草種質資源與遺傳育種 ( C161502 )
項目負責人趙雁 負責人職稱副教授 依托單位云南農業大學
資助金額39.00
萬元
項目類別地區科學基金項目 研究期限2017 年 01 月 01 日 至
2020 年 12 月 31 日
中文主題詞德欽紫花苜蓿;高溫脅迫;根部;代謝組;轉錄組
英文主題詞Medicago sativa Linn. ‘Deqin’;Heat stress;Root;Metabonome;Transcriptome

摘要

中文摘要 德欽紫花苜蓿野生資源成片分布于云南省金沙江干熱河谷地帶,具有較強的耐干熱性。本研究團隊前期研究表明,在高溫脅迫下,德欽紫花苜蓿葉片特異性耐熱與其葉片四個抗氧化酶同工酶(SOD2、POD4、POD7和APX3)活性增強和6個逆境蛋白(Hsp70、Hsp18.2、Le-Hsp17.6、DnaJ、DnaK和NEFs)上調有關,受ABA、CTK和IAA共同調控。與葉片相比,根部在土溫高的環境中對植物的存活與否起關鍵作用,但高溫脅迫下德欽紫花苜蓿根部應答調控機制未見報道。本項目以德欽紫花苜蓿盆栽苗為材料,在不同的熱激處理下,分析其根部質膜ATPase活性等生理變化和代謝產物積累特征,篩選3-5個標志物;進行根部轉錄組分析,篩選1-3個根部抗熱關鍵基因;采用原核表達和qRT-PCR方法進行抗熱特異基因的驗證。研究結果將深入解析德欽紫花苜蓿根部應答高溫脅迫的調控機制,為紫花苜蓿抗熱分子育種奠定基礎。
英文摘要 The wild resources of Medicago sativa Linn. ‘Deqin’ (hereinafter referred to as the ‘Deqin’), with strong drought and heat tolerance, distributed in dry-hot valley zone of Yangzi River, Yunnan province, China. Our previous research result shows that the special thermotolerance of ‘Deqin’ was closely related to its four strong antioxidant isozymes (SOD2, POD4, POD7 and APX3) activities and six up-regulated heat induced molecular chaperones (Hsp70, Hsp18.2, Le-Hsp17.6, DnaJ, DnaK and NEFs) , which were regulated by ABA, CTK and IAA. Compared with leaves, roots play critical roles in plant survival in environments with high soil temperature. However, there is no report about the regulation mechanisms of ‘Deqin’ roots in response to heat stress. Pot plants filled with loamy soil of ‘Deqin’ which are exposed to high temperature will be used as testing material in this project. The researchers will analyze the physiological change, ion fluxes and ATPase activity of plasma membrane in ‘Deqin’ roots. In addition, we will analyze the accumulation of metabolites in ‘Deqin’ roots and screen out 3 to 5 metabolite markers. And then we analyze the transcriptome of ‘Deqin’ roots, screen out 1 to 3 heat-resistance key gene (s) and combine with the result of physiology analysis and metabolites accumulation. Finally we validate these key genes on heat resistant in ‘Deqin’ roots by prokaryotic expression and qRT-PCR. All the results will give us a further understand about the regulation mechanism of ‘Deqin’ in response to heat stress, and lay the foundation for heat-resistance alfalfa varieties by molecular breeding.
結題摘要

成果

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